CGM - Department Cellular Dynamics and Development
Intercellular signaling and morphogenesis
Group leader: Anne-Marie PRET
Last update: 16-Nov-2011
Whole Drosophila ovary triple stained with DAPI (blue) to reveal all nuclei, anti-Vasa (pink) antibodies to reveal early germ cells and phalloïdin to reveal actin filaments (green)
L'équipe
François Agnès, Teacher/Researcher, Univ. Paris-Sud 11
Mathieu Bartoletti, Thesis student
Antoine Borensztejn, Thesis student
Fabienne Chalvet, Teacher/Researcher, Univ. Paris-Sud 11
Sophie Netter, Teacher/Researcher, UVSQ
Anne-Marie Pret, Professor, UVSQ
Laurent Théodore, Professor, Univ. Paris-Sud 11
Our address
CNRS - Centre de Génétique Moléculaire - UPR 3404
Avenue de la Terrasse - Bât. 26
91198 GIF-SUR-YVETTE Cedex
FRANCE
Tél. : 33 (0)1 69 82 31 46 ou 31 89
Fax : 33 (0)1 69 82 43 86
Research interests
Our group joined the Development Department at the Molecular Genetics Center in February 2005 with support from a 3-year young researcher grant from the CNRS (Centre National pour la Recherche Scientifique) and a grant for newly-installed groups from the FRM (Fondation pour la Recherche Medicale). We use Drosophila oogenesis as a model system to study the regulation of morphogenesis by intercellular signaling pathways. Our work is centered around 3 major themes :
• Role of the Hedgehog signaling pathway for the coordinated regulation of proliferation and differentiation of somatic and germline cells in the adult and pupal ovary ;
• Identification of new genes involved in soma-germen interactions early stages of oogenesis using enhancer-trap and UAS-Gal4 misexpression screens ;
• Genetic analysis of programmed cell death by apoptosis in the somatic polar cell lignage for the selection of 2 pairs of polar cells per ovarian follicle.
Selected publications
Khammari, A., Agnès, F., Gandille, P., Pret, A-M. (2011) Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1. Cell Death Differ, 18 (5) 793-805.
Gandille, P., Narbonne-Reveau K., Boissonneau, E., Randsholt, N., Busson, D, Pret, A-M. (2010) Mutations in the polycomb group gene polyhomeotic lead to epithelial instability in both the ovary and wing imaginal disc in Drosophila. PLoS One, 5 (11) e13946.
Tricoire, H., Battisti, V., Trannoy, S., Lasbleiz, C., Pret, A.-M. and Monnier, V. (2009) The steroid hormone receptor EcR finely modulates Drosophila lifespan during adulthood in a sex-specific manner. Mech Ageing Dev, 130 (8) 547-552.
Busson, D. and Pret, A.-M. (2007) UAS/Gal4 expression and useful drivers for Drosophila Hedgehog signaling. In Methods in Molecular Biology, 19 (1) 226-36, Humana Press, New Jersey USA.
Simionato, E., Barrios, N., Duloquin, L., Boissonneau, E., Lecorre, P. and Agnes, F. (2007) The Drosophila RNA-binding protein ELAV is required for commissural axon midline crossing via control of commissureless mRNA expression in neurons. Dev Biol, 301 (1) 166-77
Narbonne, K., Besse, F., Isnard, C., Busson, D. and Pret, A.M. (2006) fused is required for regulation of germline cyst mitosis and differentiation during Drosophila oogenesis. Mech. Develop. 123 :197-209
Besse, F., Busson, D. and Pret, A.-M. (2005) Hedgehog signal transduction controls germ cell-soma interactions during Drosophila ovarian morphogenesis Dev. Dynamics 234 : 422-431.
Narbonne, K., Besse, F., Brissard, J., Pret, A.-M., and Busson, D. (2004) polyhomeotic is required for somatic cell proliferation and differentiation during ovarian follicle formation in Drosophila. Development 131 : 1389-1400.
Besse, F. and Pret, A.M. (2003) Apoptosis-mediated cell death within the ovarian polar cell lineage of Drosophila melanogaster. Development 130 : 1017-1027.
Besse, F., Busson, D. and Pret, A.-M. (2002) Fused-dependent Hedgehog signal transduction is required for somatic cell differentiation during egg chamber formation in Drosophila. Development 129 :4111-4124.
Busson, D., Brissard, J., Pret, A.-M., Tricoire, H. (2000) Utilisation des ip3 kinases pour moduler in vivo la sensibilité au stress oxydatif Brevet Européen.
Besse F. and Pret A.-M. (1999) Piwi…gène de l’immortalité ? Médecine/Sciences, 15 : 19.
To know more about A.-M. Pret research
