CGM - Department Genes Expression
Metabolism and function of RNA in the nucleus
Group leader: Domenico LIBRI
Last update : 09/01/12
Our team
Jocelyne Boulay, Assistant-Ingénieur, CNRS (1)
Jean-Baptiste Briand, Doctorant (2)
Jessie Colin, Post-doctorant (3)
Marie-Claire Daugeron, Maître de Conf., Paris-Sud11 (4)
Martina Frezzarin, Doctorante Univ. Padova (5)
François Lacroute, Prof. Emérite, UPMC (6)
Domenico Libri, Directeur de Recherche, CNRS (7)
Odil Porrua Fuerte, Post-doctorante (8)
Mathieu Rougemaille, Chargé de Recherche, CNRS (8)
Agnieszka Tudek, Doctorante (10)
Tommaso Villa, Chargé de Recherche, CNRS (11)
Our address
CNRS - UPR 3404
Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 24
91198 GIF-SUR-YVETTE Cedex
FRANCE
Tél. : 33 (0)1 69 82 38 09
Fax : 33 (0)1 69 82 38 77
Research themes
The Libri lab currently focuses on three main lines of investigation, using the powerful combination of genetics and biochemical approaches allowed by the use of bakers’ yeast Saccharomyces cerevisiae as a eukaryotic model system:
•1) We study the metabolism and function of a novel class of non-coding RNAs, the Cryptic Unstable Transcripts (CUTs) that we have recently identified working together with the groups of A. Jacquier (Institut Pasteur) and Bertrand Séraphin (CGM) . These RNAs are very unstable and cannot generally be detected in wild type yeast. However they represent a large share of “hidden” transcription (i.e. transcription without production of detectable transcripts) throughout the yeast genome. We are interested in studying the function (if any) of these RNAs and the reason why they are transcribed and degraded.
“Many thanks to Ebbe Sloth Andersen for his beautiful drawings”
•2) We study the function of protein complexes and factors involved in the assembly and subsequent export to the cytoplasm of messenger ribonucleoproteins (mRNPs). We also study the mechanisms that control the quality of mRNPs and promote discard of defective molecules to avoid that incorrect or contradictory messages are expressed.
• 3) We have recently identified and characterized a novel and evolutionarily conserved protein complex with a role in transcriptional activation in yeast and telomere maintenance. We study the mechanism(s) by which this complex affects both processes.
Main publications
(links to pdf files)
Revues
- Rougemaille, M., Libri, D. (2011) Control of cryptic transcription in eukaryotes. Adv Exp Med Biol, 702, 122-3.
- Villa, T., Rougemaille, M. and Libri, D. (2008) Nuclear quality control of RNA polymerase II ribonucleoproteins in yeast: tilting the balance to shape the transcriptome. Biochimica et Biophysica Acta, 1779 (9) 524-31.
- Rougemaille, M., Villa, T., Gudipati, R. K. and Libri, D. (2008) mRNA journey to the cytoplasm: attire required. Biol Cell, 100 (6) 327-42.
- Jensen, T. H., Dower, K., Libri, D., and Rosbash, M. (2003). Early formation of mRNP: license for export or quality control? Mol Cell 11, 1129-1138.
Articles
- Assenholt, J., Mouaikel, J., Saguez, C., Rougemaille, M., Libri, D., Jensen, TH. (2011) Implication of Ccr4-Not complex function in mRNA quality control in Saccharomyces cerevisiae. RNA, 17(10) 1788-94.
- Daugeron, M.-C., Lenstra, T.L., Frizzarin, M., El Yacoubi, B., Liu, X., Baudin-Baillieu, A., Lijnzaad, P., Decourty, L., Saveanu, C., Jacquier, A., Holstege, F.C., de Crécy-Lagard, V., van Tilbeurgh, H., Libri, D. (2011) Gcn4 misregulation reveals a direct role for the evolutionary conserved EKC/KEOPS in the t6A modification of tRNAs. Nucleic Acids Res, 39 (14) 6148-6160.
- Daugeron, M-C., Prouteau, M., Lacroute, F., Séraphin, B. (2011) The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1. Nucleic Acids Res, 39 (6) 2221-33.
- Colin, J., Libri, D., Villa, T. (2010) Sex matters in the birth of genes. Cell Res, 20 (5) 499-501.
- Libri, D. (2010) Nuclear poly(a)-binding proteins and nuclear degradation: take the mRNA and run? Mol Cell, 37 (1) 3-5.
- Rougemaille, M., Dieppois, G., Kisseleva-Romanova, E., Gudipati, R.-K., Lemoine, S., Blugeon, C., Boulay, J., Jensen, T.-H., Stutz, F., Devaux, F. and Libri, D. (2008) THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. Cell, 135 (2) 308-21.
- Assenholt, J., Mouaikel, J., Andersen, K.-R., Brodersen, D., Libri, D. and Jensen, T.-H. (2008) Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants. RNA, 14 (11) 2305-13.
- Thiebaut, M., Colin, J., Neil, H., Jacquier, A., Séraphin, B., Lacroute, F. and Libri, D. (2008) Futile cycle of transcription initiation and termination modulates the response to nucleotide shortage in S. cerevisiae. Mol Cell, 31 (5) 671-82.
- Libri, D. (2008) Quality control: Cell and the city. Biochim Biophys Acta, 1779 (9) 523.
- Hecker, A., Lopreiato, R., Graille, M., Collinet, B., Forterre, P., Libri, D. and van Tilbeurgh, H. (2008) Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplex. EMBO J, 27 (17) 2340-2351.
- Gudipati, R. K., Villa, T., Boulay, J. and Libri, D. (2008) Phosphorylation of RNA polymerase CTD dictates transcription termination choice. Nat Str Mol Biol, 15 (8) 786-94.
- Rougemaille, M., Gudipati, R. K., Olesen, J. R., Thomsen, R., Seraphin, B., Libri, D. and Jensen, T. (2007) Dissecting mechanisms of nuclear mRNA surveillance in THO/sub2 complex mutants. EMBO J, 26 (9) 2317-26.
- Thiebaut, M., Kisseleva-Romanova, E., Rougemaille, M., Boulay, J. and Libri, D. (2006). Transcription termination and nuclear degradation of cryptic unstable transcripts: a role for the nrd1-nab3 pathway in genome surveillance. Mol Cell, 23 (6) 853-64.
- Kisseleva-Romanova, E., Lopreiato, R., Baudin-Baillieu, A., Rousselle, J.-C., Ilan, L., Hofmann, K., Namane, A., Mann, C. and Libri, D. (2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex. EMBO J, 25 (15) 3576-85.
- Cerchia, L., Duconge, F., Pestourie, C., Boulay, J., Aissouni, Y., Gombert, K., Tavitian, B., de Franciscis, V., and Libri, D. (2005). Neutralizing aptamers from whole-cell SELEX inhibit the RET receptor tyrosine kinase. PLoS Biol 3, e123.
- Wyers, F., Rougemaille, M., Badis, G., Rousselle, J., Dufour, M., Boulay, J., Regnault, B., Devaux, F., Namane, A., Seraphin, B., Libri, D., and Jacquier, A. (2005). Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase. Cell 121, 725-737.
- Jensen, T., Boulay, J., Olesen, J., Colin, J., Weyler, M., and Libri, D. (2004). Modulation of transcription affects mRNP quality. Molecular Cell 16, 2, 235-244.
- Libri D, Dower K, Boulay J, Thomsen R, Rosbash M, Jensen TH.(2002) Interactions between mRNA export commitment, 3'-end quality control, and nuclear degradation. Mol Cell Biol. 22 (23) 8254-66
- Jensen TH, Boulay J, Rosbash M, Libri D. (2001) The DECD box putative ATPase Sub2p is an early mRNA export factor. Curr Biol 11 (21) 1711-5.
- Libri D, Graziani N, Saguez C, Boulay J. (2001) Multiple roles for the yeast SUB2/yUAP56 gene in splicing. Genes Dev 15 (1) 36-41.
Publications since 2000 :
