CGM - Department Cellular Dynamics and Development
Microbial infection of Drosophila: a model for
dissecting
host-pathogen interactions
Group leader: Bruno LEMAITRE
Last update : 12-Oct-2009
Team members
Audrey Boniface, Post-doctorante
Hyunsuk Lee, Stagiaire EPHE
Bruno Lemaitre, Directeur de Recherche
Nouara Lhocine, Doctorant
François Leulier, Chargé de Recherche
Mickaël Poidevin, Technicien
Isabelle Vallet-Gély, Chargé de Recherche
RNAi screening facility
Agathe Juppeau, Technicien
Bruno Lemaitre has been nominated for a professorial chair of Genetics at the "Ecole Polytechnique Fédérale de Lausanne": he is now the group leader of a new lab at EPFL.
Our address
CNRS - Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 26
91198 GIF-SUR-YVETTE Cedex
FRANCE
Phone : 33 (0)1 69 82 32 16
Telecopy : 33 (0)1 69 82 43 86
Research interests
Our group focuses on understanding mechanisms of microbial infection and corresponding host defense responses using Drosophila as a model system. Our team has established strong collaborative links with the Frédéric Boccard's team.
We are also hosting a genome-wide in vivo RNAi screening facility to identify new genes involved in four biological functions : immunity, circadian rythm, epigenetism and cell polarity. We are working in collaboration with the teams of François Rouyer (NGI- INAF, Gif-sur-Yvette), Christophe Antonievsky (Institut Pasteur, Paris) et François Schweisguth (ENS, Paris).
Key publications since 2003
Buchon, N., Poidevin, M., Kwon, H-M., Guillou, A., Sottas, V., Lee, B-L. and Lemaitre, B. (2009) A single modular serine protease integrates signals from pattern-recognition receptors upstream of the Drosophila Toll pathway. Proc Natl Acad Sci U S A, 106 (30) 12442-7.
Defaye, D., Evans, I., Crozatier, M., Wood, W., Lemaitre, B. and Leulier, F. (2009) Genetic Ablation of Drosophila Phagocytes Reveals Their Contribution to Both Development and Resistance to Bacterial Infection. J Innate Immun, in press.
Buchon, N., Broderick, N., Poidevin, M., Pradervand, S. and Lemaitre, B. (2009) Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation. Cell Host Microbe, 5 (2) 200-11.
Quevillon-Cheruel, S., Leulliot, N., Muniz, C., Vincent, M., Gallay, J., Argentini, M., Cornu, D., Boccard, F., Lemaître, B. and van Tilbeurgh, H. (2009) Evf, a Virulence Factor Produced by the Drosophila Pathogen Erwinia carotovora, Is an S-Palmitoylated Protein with a New Fold That Binds to Lipid Vesicles. J Biol Chem, 284 (6) 3552-3562.
Tang, H., Kambris, Z., Lemaitre, B. and Hashimoto, C. (2008) A Serpin that Regulates Immune Melanization in the Respiratory System of Drosophila. Dev Cell, 15 (4) 617-26.
Scherfer, C., Tang, H., Kambris, Z., Lhocine, N., Hashimoto, C. and Lemaitre, B. (2008) Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation. Dev Biol, 323 (2) 189-96.
Lhocine, N., Ribeiro, P., Buchon, N., Wepf, A., Wilson, R., Tenev, T., Lemaitre, B., Gstaiger, M., Meier, P. and Leulier, F. (2008) PIMS modulates immune tolerance by negatively regulating Drosophila innate immune signaling. Cell Host Microbe, 4 (2) 147-58.
Vallet-Gely, I., Lemaitre, B. and Boccard, F. (2008) Bacterial strategies to overcome insect defences. Nat Rev Microbiol, 6 (4) 302-13.
Leulier, F. and Lemaitre, B. (2008) Toll-like receptors-taking an evolutionary approach. Nat Rev Genet, 9 (3) 165-78.
Lemaitre, B. and Hoffmann, J. (2007) The Host Defense of Drosophila melanogaster. Annual Review of Immunology, 25 697-743.
Vodovar, N., Vallenet, D., Cruveiller, S., Rouy, Z., Barbe, V., Acosta, C., Cattolico, L., Jubin, C., Lajus, A., Segurens, B., Vacherie, B., Wincker, P., Weissenbach, J., Lemaitre, B., Médigue, C., and Boccard, F. (2006) The complete genome sequence of the entomopatogenic and metabolically versatile soil bacterium Pseudomonas entomophila. Nature Biotech 4(6):673-9.
Liehl P, Blight M, Vodovar N, Boccard F, Lemaitre B (2006) Prevalence of local immune response against oral infection in a Drosophila/Pseudomonas infection model. PLoS Pathog 2(6): e56
Zaidman-Remy A., Hervé M., Poidevin M., Pili-Floury S., Kim M-S , Blanot D., Oh B.H., Ueda R., Mengin-Lecreulx D. and B. Lemaitre. (2006) The Drosophila amidase PGRP-LB adjusts the immune response to bacterial infection, Immunity 24(4):463-73.
Kambris, K., Brun, S., Jang, I.-H., Nam, H.-J., Romeo, Y., Takahashi, K., Lee, W., Ueda, R., and Lemaitre, B. (2006). A large-scale in vivo RNAi screen identifies five new serine proteases required for Toll activation during the Drosophila immune response. Current Biology 6(8):808-13. suppl, table
Vodovar N. Vinals M., Liehl P., Basset A., Degrouard J., Spellman P., Boccard F. and B. Lemaitre (2005) Drosophila host defense after oral infection by a new entomopathogenic Pseudomonas species. Proc Natl Acad Sci U S A. 102:11414-9.
Leulier F., Parquet C., Pili-Floury S., Ryu J.-H., Caroff M., Lee W.-J., Mengin-Lecreulx D. and Lemaitre B. The Drosophila immune system detects both Gram-positive and Gram-negative bacteria through specific peptidoglycan recognition. (2003) Nature immunology 4(5):478-84.
To know more about B. Lemaitre's lab
About our research interests:
• Regulation of antimicrobial peptide gene expression by the Toll and Imd pathways
• Genomic analysis of Drosophila immune responses
• Host-pathogen interactions in Drosophila in collaboration with Frédéric Boccard (CGM)
