CGM - Department Regulation and Compartmentalization of Cellular Functions
Biogenesis of bioenergetic complexes in the facultative
photosynthetic proteobacterium, Rubrivivax gelatinosus
Team leader: Chantal ASTIER
Last update: 16-Feb-2011
Our team
Chantal Astier, Professor, Univ. Paris-Sud 11
Asma Azzouzi, PhD student
Marie-Line Bourbon, Technician
Anne Durand, Associate prof., Paris-Sud 11
Sylviane Liotenberg, Associate prof., Paris-Sud 11
Soufian Ouchane, Scientist, CNRS
Anne Soisig Steunou, Scientist, CNRS
Our address
CNRS - Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 26
91198 GIF-SUR-YVETTE Cedex
FRANCE
Phone : 33 (0)1 69 82 31 37
Telecopy : 33 (0)1 69 82 32 30
Research themes
Our group uses the beta-proteobacterium Rubrivivax gelatinosus as a model to study respiration and photosynthesis. This bacterium adaptates quickly its metabolism in function of the environmental conditions and particularly the oxygen tension in the medium. Our experiments combine molecular genetics, structural biochemistry and biophysics.
We have identified most of the genes involved in photosynthesis and respiration. We are studying their expression and their regulation by oxygen. We are also investigating the role of the respiration in photosynthesis.
Recently we have identified many assembly genes of the cytochrome c oxidases that show high similarities to those identified in eukaryotes, and started a project with the aim to define the role of these genes during the biogenesis of these oxidases.
Key points for the last years
Discovery of a second Rieske protein,
Identification of two transcriptional regulators sensitive to the oxygen tension,
• PpsR: involved in both negative and positive control of the photosynthetic genes,
• FnrL: a global Regulator of Photosynthesis and Respiration,
Identification of terminal oxidases (cbb3, caa3 and bd) and their crucial role to induce and control photosynthesis,
Evidence for domain movement of the CcoO monoheme subunit of the cbb3 -oxidase from Rubrivivax gelatinosus. (coll. BIP-Marseille),
Identification of assembly genes of cytochrome c oxidases that show high similarities to those identified in eukaryotes.
Recent publications
- Jensen, SI., Steunou, AS., Bhaya, D., Kühl, M., Grossman, AR. (2011) In situ dynamics of O(2), pH and cyanobacterial transcripts associated with CCM, photosynthesis and detoxification of ROS. ISME J, 5 (2) 317-28.
- Khalfaoui Hassani, B., Steunou, AS., Liotenberg, S., Reiss-Husson, F., Astier, C., Ouchane, S. (2010) Adaptation to oxygen: Role of terminal oxidases in photosynthesis initiation in the purple photosynthetic bacterium, Rubrivivax gelatinosus. J Biol Chem, 285 (26) 19891-9.
- Khalfaoui Hassani, B., Astier, C., Nitschke, W., Ouchane, S. (2010) CtpA a copper-translocating P-type ATPase involved in the biogenesis of multiple copper-requiring enzymes. J Biol Chem, 285 (25) 19330-7.
- Liotenberg, S., Steunou, A.S., Picaud, M., Reiss-Husson, F., Astier, C., Ouchane, S. (2008). Organization and expression of photosynthesis genes and operons in anoxygenic photosynthetic proteobacteria. Env. Microbiol, 10 (9) 2267-76.
- Ducluzeau, A.L., Ouchane S., Nitschke W. (2008). cbb3 Oxidases are an Ancient Innovation of the Domain Bacteria. Mol Biol Evol, 25 (6) 1158-1166.
- Ouchane, S., Picaud, M., Therizols, P., Reiss-Husson, F. and Astier, C. (2007) Global Regulation of Photosynthesis and Respiration by FnrL. The first two targets in thetetrapyrrole pathway J Biol Chem, 282 (2) 7690-9
- Ouchane, S., Nitschke, W., Bianco, P., Vermeglio, A., and Astier, C. (2005). Multiple Rieske genes in prokaryotes: exchangeable Rieske subunits in the cytochrome bc-complex of Rubrivivax gelatinosus. Mol Microbiol 57, 261-275.
- Steunou, A., Astier, C., and Ouchane, S. (2004). Regulation of photosynthesis genes in Rubrivivax gelatinosus: transcription factor PpsR is involved in both negative and positive control. J Bacteriol 186, 3133-3142.
- Ouchane, S., Steunou, A., Picaud, M., and Astier, C. (2004). Aerobic and anaerobic Mg-protoporphyrin monomethyl ester cyclases in purple bacteria: a strategy adopted to bypass the repressive oxygen control system. J Biol Chem 279, 6385-6394
- Pinta, V., Picaud, M., Reiss-Husson, F. and Astier, C. (2002) Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved, putative binuclear-iron-cluster-containing oxygenase subunit involved in aerobic oxidative cyclization of Mg-protoporphyrin IX monomethylester J Bact, 184, 746-753.
- Ouchane, S., Agalidis, I. and Astier, C. (2002) Natural resistance to inhibitors inubiquinol-cytochrome c reductase from Rubrivivax gelatinosus. Sequence and functional analysis of the bc1 complex J Bact, 184, 3815-3822.
All the publications of the team since 1995
