CGM - Department Dynamics & Stability of Genomes
Programmed genome rearrangements - Mechanisms and regulation
Group leader: Mireille BÉTERMIER
Last update: 05-Oct-2011
The lab
Mireille Bétermier, CNRS Researcher
Julien Bischerour, CNRS Researcher
Emeline Dubois, Post-doctoral fellow
Antoine Marmignon, PhD student
Nathalie Mathy, Engineer
Vinciane Régnier, Associate Prof., Univ. Paris-Diderot
How to contact us
CNRS - Centre de Génétique Moléculaire
Avenue de la Terrasse - Bât. 26
91198 GIF-SUR-YVETTE Cedex
FRANCE
Phone: 33 (0)1 69 82 31 64
Telecopy: 33 (0)1 69 82 31 81
Research interest
We study the molecular mechanisms involved in developmentally programmed DNA rearrangements, using the ciliate Paramecium tetraurelia as a model system. Recent advances from our laboratory have demonstrated that thousands of short germ line DNA sequences, the IESs (Internal Eliminated Sequences) are removed precisely from genes and from other - non coding - genomic regions, within a define time-window during the assembly of the somatic genome. We have also shown that double strand breaks are introduced at IES ends to initiate their excision.
Our group joined the CGM in March 2006, with a financial support from the CNRS (ATIP program in Genetics). Our undergoing work is focused on the identification of protein partners involved in IES excision, using molecular, cellular and genomic approaches.
Selected publications
- Kapusta, A., Matsuda, A., Marmignon, A., Ku, M., Silve, A., Meyer, E., Forney, JD., Malinsky, S., Bétermier, M. (2011) Highly Precise and Developmentally Programmed Genome Assembly in Paramecium Requires Ligase IV-Dependent End Joining. PLoS Genet, 7 (4) :e1002049.
- Nowak, J. K., Gromadka, R., Juszczuk, M., Jerka-Dziadosz, M., Maliszewska, K., Mucchielli, M. H., Gout, J. F., Arnaiz, O., Agier, N., Tang, T., Aggerbeck, L. P., Cohen, J., Delacroix, H., Sperling, L., Herbert, C. J., Zagulski, M., Betermier, M. (2011) A functional study of genes essential for autogamy and nuclear reorganization in Paramecium. Eukaryot Cell, 10 (3) 363-72.
- Bouhouche, K., Gout, J-F., Kapusta, A., Bétermier, M., Meyer, E. (2011) Functional specialization of Piwi proteins in Paramecium tetraurelia from post-transcriptional gene silencing to genome remodelling. Nucleic Acids Res,39 (10) 4249-4264.
- Arnaiz, O., Gout, J-F., Bétermier, M., Bouhouche, K., Cohen, J., Duret, L., Kapusta, A., Meyer, E. and Sperling, L. (2010) Gene expression in a paleopolyploid: a transcriptome resource for the ciliate Paramecium tetraurelia. BMC Genomics, 11:547.
- Beisson, J., Bétermier, M., Bré, M.-H., Cohen, J., Duharcourt , S., Duret, L., Kung, C., Malinsky, S., Meyer, E., Preer, J.-R. and Sperling, L. (2010) Paramecium tetraurelia: The Renaissance of an Early Unicellular Model. CSH Protoc, 2010 (1) pdb.emo140.
Complements to this publication may be found at the following URL: http://paramecium.cgm.cnrs-gif.fr/parawiki/Protocols
- Baudry, C., Malinsky, S., Restituito, M., Kapusta, A., Rosa, S., Meyer and E., Bétermier, M. (2009). PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements in the ciliate Paramecium tetraurelia. Genes & Dev, 23 (21) 2478-83.
- Lepère, G., Bétermier, M., Meyer, E. and Duharcourt, S. (2008) Maternal noncoding transcripts antagonize the targeting of DNA elimination by scanRNAs in Paramecium tetraurelia. Genes Dev, 22 (11) 1501-12.
- Gratias, A., Lepère, G., Garnier, O., Rosa, S., Duharcourt, S., Malinsky, S., Meyer, E. and Bétermier, M. (2008) Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites. Nucleic Acids Res, 36 (10) 3244-51.
- Aury, J-M., Jaillon, O., Duret, L., Noel, B., Jubin, C., Porcel, BM., Ségurens, B., Daubin, V., Anthouard, V., Aiach, N., Arnaiz, O., Billaut, A., Beisson, J., Blanc, I., Bouhouche, K., Câmara, F., Duharcourt, S., Guigo, R., Gogendeau, D., Katinka, M., Keller, A-M., Kissmehl, R., Klotz, C., Koll, F., Le Mouël, A., Lepère, G., Malinsky, S., Nowacki, M., Nowak, J-K., Plattner, H., Poulain, J., Ruiz, F., Serrano, V., Zagulski, M., Dessen, P., Bétermier, M., Weissenbach, J., Scarpelli, C., Schächter, V., Sperling, L., Meyer, E., Cohen, J., & Wincker, P. (2006) Global trends of whole-genome duplications revealed by the ciliate Paramecium tetraurelia. Nature, 444(7116):171-178.
- Bétermier, M. (2004). Large-scale genome remodelling by the developmentally programmed elimination of germ line sequences in the ciliate Paramecium. Res. Microbiol. 155 : 399-408.
- Gratias, A., Bétermier, M. (2003). Processing of double-strand breaks is involved in the precise excision of Paramecium IESs. Mol. Cell. Biol. 23 : 7152-7162.
- Bétermier, M., Duharcourt, S., Seitz, H., Meyer, E. (2000) Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequences. Mol. Cell. Biol. 20 : 1553-1561.
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